Elisa Test Kit fan Aflatoxin B1
Grutte doses fan aflatoxinen liede ta akute fergiftiging (aflatoxicosis) dy't libbensgefaarlik wêze kin, meastentiids troch skea oan 'e lever.
Aflatoxine B1 is in aflatoxine produsearre troch Aspergillus flavus en A. parasiticus.It is in tige krêftige karzinogen.Dizze karsinogenyske krêft ferskilt oer soarten mei guon, lykas rotten en apen, skynber folle gefoeliger as oaren.Aflatoxin B1 is in mienskiplike kontaminant yn in ferskaat oan fiedings, ynklusyf pinda's, katoensiedmiel, mais en oare granen;likegoed as bistefoer.Aflatoxin B1 wurdt beskôge as de meast giftige aflatoxine en it is tige belutsen by hepatocellular carcinoma (HCC) yn minsken.Ferskate sampling- en analytyske metoaden, ynklusyf tinne-laach chromatografy (TLC), hege prestaasjes floeistofchromatografy (HPLC), massa spektrometrie, en enzyme-keppele immunosorbent assay (ELISA), ûnder oaren, binne brûkt om te testen op aflatoxine B1 kontaminaasje yn iten .Neffens de Food and Agriculture Organization (FAO) waarden de wrâldwide maksimale tolerearre nivo's fan aflatoxine B1 rapportearre te wêzen yn it berik fan 1-20 μg / kg yn iten, en 5-50 μg / kg yn dieet fee feed yn 2003.
Details
1.Elisa Test Kit foar Aflatoxin B1
2. Kat.KA07202H-96wells
3. Kit komponinten
● Microtiter plateprecoated mei antigen, 96 wells
● Standert oplossing × 6 flesse (1 ml / flesse)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzym konjugaat 7ml……………………………………………………………………………………… reade kap
● Antibody-oplossing 7ml ................................................... ...........................................................................................
● Substraat A 7ml………………………………………………………………………………………………….. wite kap
● Substraat B 7ml……………………………………………………………………………………………………… reade kap
● Stop oplossing 7ml……….……….………………………………………………………………………………………………………………………………………………………………………………………………………………………………
● 20 × konsintrearre wask oplossing 40ml ………………………………………………… transparante kap
● 2×konsintrearre ekstraksjeoplossing 50ml……………………………………………………… blauwe kap
4.Sensitiviteit, krektens en krektens
Gefoelichheid: 0.05ppb
5.Detection limyt
Eetbare oaljemonster ................................................... ........................................................................ ........................0.1ppb
Peanut ................................................... ........................................................................ .........................0.2ppb
Cereal ........................................................................ ........................................................................ ...........................0.05ppb
Krektens
Eetbare oaljemonster ................................................... ........................................................................ ...................80±15%
Peanut ................................................... ........................................................................ .....................80±15%
Cereal ........................................................................ ........................................................................ .....................80±15%
Krektens:Fariaasjekoëffisjint fan 'e ELISA-kit is minder dan 10%.
6.Cross Rate
Aflatoxine B1··················· 100%
Aflatoxine B2·······················81 ,3%
Aflatoxine G1·······················62%
Aflatoxine G2·······················22,3%